Analysis of Genetic Defect that Prevents AIDS :: Biology Gene HIV

IntroductionA number of recent studies plant overwhelming evidence that there is a genetic factor which presents itself in the gentle host, and is critical to the ineffectivity of the In Vitro transmission of HIV-1. This genetic factor is an unmarried defective CKR-5 (also known as CCR-5) allele containing a 32-base pit undercut in a region of the gene opposite to the second extracellular loop of the receptor. This defect faults the normal expression of the CKR-5 which is the co-receptor for the macrophagic tropic strains of HIV-1. Through a study of 1252 high-risk homosexual men in the Chicago area, indications were that no members of the HIV-infected caucasians were homozygous for the mutation, and the frequency of heterozygotes was 35% lower than in the general population. unmatched percent of the people with Western European decent were found to be homozygous for the defect while about 20% were found to heterozygous. This shows that the 32-base pair deracination of the CK R-5 allele is a very important factor in the HIV-1 transmission and pathogenesis.The 32-base pair deletion and its implications is a new and exciting pick up in the rhelm of AIDS and HIV research. Every day new findings are being published dealing with the topic. As research progresses, is is possible that unity day a genetic cure for this type of HIV transmission system will be found.ResearchThese findings from experiments conducted in the initial research conduct to the discovery that HIV-1 needs the coreceptor missing in homozygous individuals to infect CD4+ cells. purpose CKR-5 transcripts from EU Cells do not encode an active coreceptor.Tests were conducted on human embryonic kidney cells infected with CD4 and CKR-5 expression vectors. The vectors were then derived and rendered inactive instead of producing vectors that monitored the insertion of the macrophagic-tropic virus form.Finding EU CKR-5 RNA contains a 32-base coding era deletion.A nucleotide sequence of the EU2, EU3, and a normal donor were examine by a set of primers. The 32-base pair deletion was found in the EU2 and EU3 individuals.Finding A defective CKR-5 is encoded in the genomic DNA and is inherited.The firsts tests conducted were aimed at find out if the deletion was inherited or a mutation. The presence of the CKR-5 allele indicated that the deletion was in the genomic DNA and that it was not sex-linked.Finding The EU2 and EU3 individuals were homozygous for the CKR-5 deletion.The previous analysis suggested that the deletion was either homozygous or heterozygous.